OP0195 PLASMA CHECKPOINT PROTEIN LEVELS AND GALECTIN-9 IN JUVENILE SYSTEMIC LUPUS ERYTHEMATOSUS

Background: Systemic Lupus Erythematosus (SLE) is the prototype for a systemic autoimmune disease. SLE disease of adaptive immune system where T lymphocyte dysfunction has an important role as well. Checkpoint proteins have become topic in study Tcell. The balance cell co-stimulatory and co-inhibitory receptors regulation response. Objectives: In this we aimed to assess checkpoint childhood patients Methods: Fourty-nine jSLE together with 15 age- gender- matched controls were included. Clinical features, activity scores laboratory parameters recorded both retrospectively at time samplimg. Patient samples collected their last visit, Plasma CD25 (IL-2Rα), 4-1BB, B7.2 (CD86), TGF-β1, CTLA-4, PD-L1, PD-1, Tim-3, LAG- 3, Galectin-9 levels studied by cytometric bead-based multiplex assay panel according manufacturer’s instruction (LEGENDplex HU Immune Panel 1 (10-plex); catalogue number 740962, Biolegend) analysed Novocyte 3005 flow cytometer. Two-step cluster analysis procedure was conducted over chosen 7 symptom status 3 clusters final Results: A total 49 (71.4% female) diagnosed SLICC criteria healthy (73.3% included study. mean age 17.7 ± 2.6 years 13.3±1.4 years. median duration 5.7 At sampling fifteen had SLEDAI score zero (no activity), 21 them between 1-5 (mild 6-10 (moderate 5 11-19 (high >20 (very high activity). PD-L1 significantly higher patients. Other IL-2Rα also but did not reach statistical significance. There significant correlations IL-2Rα, PDL1. (Figure 1) three clinical clusters: Cluster no major organ involvement, 2 predominantly haematological involvement (n=16) (n=11) renal involvement. different among these clusters. Figure 1. Table Demographic, Laboratory Features Patients (n=49) Female gender, n (%) 35 (%71.4) Mean diagnosis, (mean±SD) 12.5±3.3 inclusion, 17.7±2.6 Duration illness, (median/IQR) (3.0-7.0) Active study, Renal 14 (28.5) Skin 11 (22.4) Musculoskeletal 9 (18.3) Hematologic Neurologic (2.0) Serositis findings (median/minimum-maximum) Hemoglobin, gr/dl 12.7 (7.3-18.3) WBC, /mm 6.900 (2.600-26.200) Platelet, 252.000 (39.000-529.000) Erythrocyte sedimentation rate, mm/hour 10 (1-71) C-reactive protein, mg/dl 0.1 (0-21.1) Complement 87.6 (25.4-186.0) 4, 15.3 (0-45.8) Anti-ds DNA, IU/ml 12.8 (0-741.7) 4.69±6.69 Conclusion: Our data supports that Galectin are good markers SLE. We need larger series evaluate differences failed show correlation except References: [1]Sharabi Tsokos GC. metabolism: new insights lupus erythematosus pathogenesis therapy. Nature reviews Rheumatology 2020; 16: 100-112 [2]Nishimura H, Nose M, Hiai et al. Development lupus-like diseases disruption PD-1 gene encoding ITIM motif-carrying immunoreceptor. Immunity 1999; 11: 141-151. [3]McKinney EF, Lee JC, Jayne DR, T-cell exhaustion, co-stimulation outcome autoimmunity infection. 2015 [4]Wherry EJ Kurachi M. Molecular cellular into exhaustion. Nat Rev Immunol 2015; 15: 486-499 Disclosure Interests: None declared